Introduction to Quantitative Analysis of Peptides and Proteins by LC–MS Technologies

תאריך: 8, 9 מרץ 2015
מיקום הקורס: משרדי ביופורום, גולדה מאיר 3, נס ציונה. קומה 4
מידע כללי
משך הפעילות: 2 ימי לימודים מלאים
שעות:  09:00-17:00
עלות: 4,300 ש"ח + מע"מ
נקודות זכות: 2
מק''ט: 15910
שפת הקורס: אנגלית
על הקורס

This two-day course provides a concise introduction into the quantitative analysis of peptides and proteins in (complex) biological matrices. As such, the course is useful to researchers working in the fields of both (clinical) biomarker analysis and bioanalysis of therapeutic proteins. As an introductory course, attention is paid to all relevant background aspects, including sample pretreatment, chromatography of peptides and proteins, and mass spectrometry of both proteins and peptides. Relevant workflows in quantitative analysis are outlined and illustrated with application examples.

קהל היעד

Researchers involved or soon becoming involved in the quantitative analysis of peptides and proteins (clinical biomarkers or therapeutic proteins) using LC–MS technologies, wanting to extend their knowledge and understanding.

Introduction to Quantitative Analysis of Peptides and Proteins by LC–MS Technologies
התועלת שתופק

Insight in basic aspects of quantitative analysis of proteins
Separation of proteins and peptides
Mass spectrometry of proteins and peptides
Illustration of workflow in quantitative analysis using LC–MS

השאר פרטים למידע נוסף
  • שם מלא
  • דוא''ל
  • טלפון
  • הערות
  • שלח
תכני הקורס

Day 1

09:00  Introduction. What are proteins? Structure and physicochemical properties.
10:15  Coffee Break
10:30  Quantitative analysis of proteins. Challenges and general workflow.
12:30  Lunch
13:30  Liquid chromatography of proteins and peptides.
15:15  Coffee Break
15:30  Mass spectrometry of proteins. Issues in protein characterization.
17:00  End of the first day
Day 2

09:00  Mass spectrometry of peptides. Electrospray and MS–MS.
10:15  Coffee Break
10:30  Sample pretreatment methods for peptides and proteins
12:30  Lunch
13:30  Principles of quantitative analysis of proteins in biological matrix
15:30  Coffee Break
15:45  Application examples and future directions.
17:00  End of the Course

אודות המרצה

Dr. Wilfried Niessen

Dr. Wilfried Niessen has more than 20 years of experience as a researcher and project manager in the field of liquid chromatography - mass spectrometry. Since 1996, he worked as an independent consultant for his company hyphen MassSpec. In 2002, he was appointed (part-time) extraordinary professor in bioanalytical mass spectrometry at the Faculty of Science of the Vrije Universiteit in Amsterdam.
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