Strategies in the identification of drug metabolites using LC-MS technologies


תאריך: יפורסם בהמשך
מיקום הקורס: כפר המכביה, רמת גן
מידע כללי
משך הפעילות: 2 ימי לימודים מלאים
שעות:  09:00-17:00
עלות: 4,300 ש"ח + מע"מ
נקודות זכות: 2
מק''ט: 17257
שפת הקורס: אנגלית
על הקורס

This course provides an overview of the current state-of-the-art in the identification of drug metabolites using combined liquid chromatography–mass spectrometry (LC–MS–MS) technologies. The course discusses on strategies in metabolite identification, illustrates these strategies with typical examples, and provides interpretation exercises to deal with the strategies yourself. Attention is paid to both Phase I and Phase II metabolites, whereas a separate session is devoted to strategies directed at the detection and identification of reactive metabolites

קהל היעד

  • Researchers involved in drug metabolite identification within pharmaceutical industry or elsewhere, with at least 6 months experience in LC–MS.
  • People who want to extend their knowledge and understanding of mass spectrometry
התועלת שתופק

  • Overview of current state-of-the-art in metabolite identification using LC–MS
  • Insight in fragmentation reactions of even-electron ions
  • Understand background of MetID software tools
  • Insight in strategies for structure elucidation of drug metabolites
  • Identification of Phase I and II metabolites. Reactive metabolites
השאר פרטים למידע נוסף
  • שם מלא
  • דוא''ל
  • טלפון
  • הערות
  • שלח
תכני הקורס

Day 1

  9:00

General introduction. General workflow in metabolite profiling and identification. Brief introduction to LC–MS and MS instrumentation.

10:30

Coffee Break

10:45

General aspects of fragmentation of even-electron positive ions. Fragmentation according to and against the parity rule. Basic fragmentation mechanisms. Exercises.

12:30

Lunch

13:30

Strategies for detection and structure elucidation of metabolites using LC–MS–MS. 1. Phase I metabolism. Mass shifts in complementary fragments and profile groups to identify metabolites. Exercises.

15:00

Coffee Break

15:15

Strategies for detection and structure elucidation of metabolites using LC–MS–MS. 2. Phase I metabolism. Data-dependent LC–MS analysis: potentials and limitations. Scan modes in MS–MS. Additional technologies and experiments. Exercises.

17:00

End of the first day.

 

Day 2

  9:00

Fragmentation of even-electron ions: Interpretation of MS–MS spectra. Illustrative examples of interpreting MS–MS spectra. Software tools in predicting and interpreting fragmentation. Exercises.

10:30

Coffee Break

10:45

Strategies for detection and structure elucidation of metabolites using LC–MS–MS. 3. Accurate-mass determination. Potentials and limitations. Practical aspects. Predicting elemental compositions. Mass-defect filtering. Exercises.

12:30

Lunch

13:30

Strategies for detection and structure elucidation of metabolites using LC–MS–MS. 4. Phase II metabolism. Strategies for detection and structure elucidation of phase II metabolites using LC–MS–MS. Mass shifts and typical fragmentation of conjugates. Exercises.

15:00

Coffee Break

15:15

Strategies for detection and structure elucidation of metabolites using LC–MS–MS. 5. Reactive metabolites. Strategies for detection and structure elucidation of reactive metabolites using LC–MS–MS. Glutathione conjugates and cyano-trapping. Exercises.

17:00

End of the second day and end of the course.

אודות המרצה

Dr. Wilfried Niessen

Dr. Wilfried Niessen has more than 20 years of experience as a researcher and project manager in the field of liquid chromatography - mass spectrometry. Since 1996, he worked as an independent consultant for his company hyphen MassSpec. In 2002, he was appointed (part-time) extraordinary professor in bioanalytical mass spectrometry at the Faculty of Science of the Vrije Universiteit in Amsterdam.
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